High-throughput method for detecting five mutation sites of thalassemia gene and special reagent set thereof

The invention discloses a high-throughput method for detecting five mutation sites of thalassemia gene and a special reagent set thereof. The special reagent set comprises a set of probes and a set ofprimers. The set of probes consists of 11 probes shown in SEQ ID NO: 11 to SEQ ID NO: 19. The set of primers consists of 10 primers shown in SEQ ID NO: 1 to SEQ ID NO: 10. By adopting the method provided by the invention, the identification of the mutation types of the five mutation sites of the thalassemia gene can be realized quickly, and the method has good specificity, high flux and simple operation, and the accuracy rate reaches 100%. The invention has important application value. 本发明公开了高通量检测地中海贫血基因的5个突变位点的方法及其专用成套试剂。成套试剂包括成套探针和成套引物。成套探针由序列11至序列19所示的11个探针组成。成套引物由序列1至序列10所示的10个引物组成。采用本发明提供的方法，可以快速实现对地中海贫血基因的5个突变位点突变类型的鉴定，并且特异性好、通量高、操作简单，准确率达到100％。本发明具有重要的应用价值。