A method for detecting ploidy of watermelon

The invention discloses a method for detecting the ploidy of watermelon. The steps include preparing protoplasts, extracting total DNA, and detecting DNA concentration by quantitative PCR. Protoplastsare isolated from leaves of watermelon with different ploidy. Total DNA is extracted from the same a...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN WEI, HUANG XING, SUN YUHONG, XIONG JIANSHUN, ZENG HONGXIA, LI QIYOU, ZHANG NA, TANG MI, BI QIURONG, REN JIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for detecting the ploidy of watermelon. The steps include preparing protoplasts, extracting total DNA, and detecting DNA concentration by quantitative PCR. Protoplastsare isolated from leaves of watermelon with different ploidy. Total DNA is extracted from the same amount of protoplasts. According to the 5S rDNA sequence of watermelon, specific primers are designed for quantitative PCR. The standard curve of Ct values and DNA concentrations is plotted with different DNA concentrations. The concentration of Ct is converted and ploidy is calculated according toDNA concentration of different ploidy of watermelon. The method has low requirements for samples, and other plant tissues other than fruits and seeds with more nutrients can be separated from protoplasts. Fluorescence quantitative polymerase chain reaction (FQ-PCR) instrument with low requirement and low cost can meet the demand without purchasing relatively expensive Flow Cytometry (FCM). The accuracy of the method is sim