Rabbit sacculus rotundus antibacterial peptide and preparation method thereof

The invention provides a preparation method for a rabbit sacculus rotundus antibacterial peptide, belonging to the technical field of biomedicine. The preparation method comprises the following steps:homogenizing rabbit sacculus rotundus, mixing obtained rabbit sacculus rotundus homogenate with an acetic acid solution, and carrying out leaching for 12 to 14 h at 2 to 5 DEG C to obtain a leachingliquor; subjecting the leaching liquor to repeated freezing-thawing 4-6 times to obtain a frozen-thawed solution; subjecting the frozen-thawed solution to multi-layer filtration, microfiltration and ultrafiltration to obtain an ultrafiltrate; and adjusting the pH value of the ultrafiltrate to 6.5-7.2 so as to obtain the rabbit sacculus rotundus antibacterial peptide. With the preparation method ofthe invention, the content of polypeptide in the rabbit sacculus rotundus antibacterial peptide reaches 1.0 mg/ml or more, and the stimulation index of the rabbit sacculus rotundus antibacterial peptide is 1.10 or more; that i