Method for determining cholesterol concentration

The invention discloses a method for determining cholesterol concentration. The method comprises the following steps: (1), transferring the cholesterol solution to-be-test for centrifugation, after centrifuging, adding a 95% ethanol and a 50% KOH solution to perform saponification in a hot water bath; (2), after cooling to the room temperature, adding a petroleum ether, and performing shaking andextracting in a constant-temperature water bath shaker; (3), transferring all substances to a separatory funnel, washing with the 95% ethanol, performing standing for a liquid separation, and collecting the petroleum ether layer in a beaker; (4), evaporating an organic reagent in a water bath in a fuming cupboard; (5), dissolving the residue with an absolute ethyl alcohol, and performing ultrasonic dissolution in a hot water bath; (6), performing constant volume to obtain a cholesterol dissolving solution; (7), drawing a cholesterol standard curve; (8), transferring the cholesterol dissolvingliquid in a test tube, add