Method for extracting guaiane-type sesquiterpene compound from Cynara scolymus

The present invention provides a method of extracting a guaiane-type sesquiterpene compound from Cynara scolymus. The method comprises the following steps: pulverizing Cynara scolymus and extracting with ethanol water to obtain crude extract liquor; loading the crude extract liquor to an AB-8 macroporous resin chromatographic column for elution, carrying out chromatographic detection, collecting an eluant containing a target compound, concentrating and freeze-drying to obtain a crude extract; dissolving the crude extract in 50% methanol, loading to an RP-C18 chromatographic column for elution,carrying out chromatographic detection, collecting an eluant containing the target compound, concentrating and freeze-drying to obtain a pre-separation product; dissolving the pre-separation productin an acetonitrile aqueous solution, carrying out liquid-phase semi-preparation, collecting a flow fraction containing the target compound, concentrating and freeze-drying to a quasi-extract; dissolving the quasi-extract with