Preparation of monkey-human cell fusion maternal-fetal blood group incompatibility therapy hybrid strain

A monkey used in medicine-Preparation of human cell fusion maternal-fetal blood group incompatibility therapy hybrid strain, characterized by, using erythropoietin as inducer, ''O'' type rhesus monkeybone marrow cell positive for Rh were cultured, to increase the number of direct...

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Hauptverfasser: SU LAN, LI LANJUAN, HUANG HUANJIA, WENG BINGHUAN, CHEN ZHUOPENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:A monkey used in medicine-Preparation of human cell fusion maternal-fetal blood group incompatibility therapy hybrid strain, characterized by, using erythropoietin as inducer, ''O'' type rhesus monkeybone marrow cell positive for Rh were cultured, to increase the number of directionally developing erythroid cells, and then the cultured rhesus monkey bone marrow cells and human myeloma cells werefused into hybridized cells, Erythropoietin was use to induce that directional differentiation of the hybridized cell, hybrid cell clones were screened by HAT. The hybridization strains of Rh positivecells which can be expanded indefinitely in vitro were screened by Rh antibody. As that adsorbent aft industrialized amplification. A low-cost and safe method for that treatment of maternal-fetal blood group incompatibility hemolytic disease without plasma exchange solution without remove plasma and its beneficial ingredients is create by absorbing pathogenic Rh antibody in peripheral plasma of pregnant women with maternal