Method for extracting polysaccharides from radix millettiae speciosae

The invention discloses a method for extracting polysaccharides from radix millettiae speciosae. The method comprises the following steps: (1) the radix millettiae speciosae is dried for being crushedinto powder, and water is added for soaking the radix millettiae speciosae powder until to be soften...

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Hauptverfasser: YANG ZHENMEI, PAN YANFENG, HUANG DONGNI, JIANG MAN, MA LANHUA, LIANG JUN, HUANG FEI, QIN HONGBO, GAN GUOYONG, CAI ZHIQING, QIN JIANYOU, DENG GUANGYAN, LU JIANCHENG, CAI YANHONG, YANG CHUANCHUAN, PENG PINFENG, WEI BINGJIE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for extracting polysaccharides from radix millettiae speciosae. The method comprises the following steps: (1) the radix millettiae speciosae is dried for being crushedinto powder, and water is added for soaking the radix millettiae speciosae powder until to be softened; (2) the softened radix millettiae speciosae powder is poured into a high-pressure homogenizingmachine to be homogenized for a certain time under the condition of 6-15 MPa and then homogenized again under the condition of 20-30 MPa, homogeneous liquid is centrifuged under the condition of 3000-5000 r/min, and then supernatant is collected; (3) first microfiltration is carried out to the supernatant obtained in the step (2), an ultrafiltration membrane with molecular weigh cut-off of 5000 isselected for separation, and then filter liquor is collected; (4) ultrafiltration is carried out to the filter liquor obtained in the step (3), the ultrafiltration membrane with molecular weigh cut-off of 1000 is selected for