Paraffin slicing method for xanthoceras sorbifolia bunge cuttings and calluses thereof

The invention discloses a paraffin slicing method for xanthoceras sorbifolia bunge cuttings and calluses thereof. The method comprises fixing of materials, flushing, softening, dehydration, transparency treatment, wax immersion-embedding, slicing-sticking-spreading, dewaxing-dyeing, and sealing. During flushing, fixed stems of materials are flushed repeatedly with 50 percent ethanol for three times. During softening, the flushed stems are soaked in 5 percent by mass hydrofluoric acid, 50 percent by mass glycerol at the temperature of 50 DEG C and a softening solution for a certain period of time, wherein the softening solution is prepared by mixing 50 percent by mass alcohol and 100 percent by mass glycerol at equal volume. The method has the advantages of easiness in operation, good stability of results, convenience in carrying out a large number of basic researches, complete slices, uniform thickness, clear tissue structure, high dyeing contrast, complete cell morphology, and very high continuity and perman