Anti-beta receptor agonist cluster-specific monoclonal antibody hybridoma cell line, monoclonal antibody secreted by cell line, and applications of monoclonal antibody

The present invention relates to the field of biotechnology, and discloses an anti-beta receptor agonist cluster-specific monoclonal antibody hybridoma cell line and a monoclonal antibody secreted bythe cell line. According to the present invention, the hybridoma cell line is prepared from salbutamol artificial antigen, and the secreted monoclonal antibody can simultaneously recognize salbutamol,clenbuterol, terbutaline, brombuterol, cimbuterol and other beta-receptor agonists, and has cluster specificity; the immunoaffinity chromatography gel prepared from the monoclonal antibody and the immunoaffinity chromatography gels of other beta-receptor agonists such as ractopamine are mixed as the machine measurement pretreatment purification reagent, a variety of clenbuterol hydrochloride components in pork, pig liver and pig urine samples can be efficiently detected by combining the machine measurement pretreatment purification reagent, HPLC-FLD and LC-MS/MS, and the high accuracy and high sensitivity can be achie