Arctii radix solid streptomycete spore-producing medium and culture method

An arctii radix solid streptomycete spore-producing medium and a streptomycete culture method are disclosed. The medium includes 20-30 g/L of arctii radix, 15-25 g/L of mannitol, 15-20 g/L of agar powder and water. After arctii radix is dried and crushed, water is added, the mixture is boiled and fi...

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Hauptverfasser: ZHANG HUIZHEN, ZHAO ZHILONG, SHI XIAOWEI, KUANG PENGQUN, WANG ZHEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:An arctii radix solid streptomycete spore-producing medium and a streptomycete culture method are disclosed. The medium includes 20-30 g/L of arctii radix, 15-25 g/L of mannitol, 15-20 g/L of agar powder and water. After arctii radix is dried and crushed, water is added, the mixture is boiled and filtered, the filtrate is mixed with the mannitol and the agar powder, the pH value is adjusted with NaOH to 7.0-7.5, the preset volume is achieved, a product is poured to a plate after sterilization, and streak culture of streptomycete is performed by utilizing inoculating loops after curing. Raw materials are few, preparation is simple, and a spore-producing medium preparing method is simplified with a low cost. The amino acid content in the arctii radix is about 28% relative to the dry weight,the content of inulin is about 34% relative to the dry weight, and the arctii radix contains a plurality of trace elements. Compared with an actinomycetes medium that is a Gause medium No.1, the disclosed medium enables rapid