Kit for early detection of liver cancer and preparation method thereof

The invention discloses a kit for early detection of liver cancer and a preparation method thereof. The kit comprises a fluorescent enzyme labeling board, sample diluent, washing liquid, a negative control, a positive control, a monoclonal antibody against the liver cancer markers, a biotinylated monoclonal antibody against the liver cancers marker and a streptavidin-phycobiliprotein fluorescent probe. The kit for early detection of liver cancer reduces the step of adding chemical substrate for color development, the operation steps are simple, the sensitivity is high, and the by-products aresmall. The kit for early detection of liver cancers and preparation method thereof realizes the synthesis of streptavidin/phycobiliprotein fusion fluorescent protein by "in vivo one-step" in Escherichia coli by multi-gene combined expression. When the phycobiliprotein labeling is completed, the fusion molecule is guaranteed to have stable fluorescence properties. At the same time, the fusion protein also has good targetin