Specific marker Nagorno-Karabakh deformation microsporidia nucleic acid probe and fluorescence in-situ hybridization detection method thereof

The invention relates to a specific marker Nagorno-Karabakh deformation microsporidia nucleic acid probe and a fluorescence in-situ hybridization detection method thereof. The nucleotide sequence of the nucleic acid probe is as shown in SEQ ID NO. 1, and in-situ hybridization particularly includes the steps: fixing and then decolorizing silkworm parasitism sac tissues infected by Nagorno-Karabakhdeformation microsporidia; adding hybridization solution containing the nucleic acid probe for hybridization and cleaning the silkworm parasitism sac tissues after hybridization; marking cell nucleuses of parasitism sacs and cell nucleuses of the Nagorno-Karabakh deformation microsporidia by DAPI, and observing fluorescent probe marking results by a fluorescent microscope. The nucleic acid probe is high in combination sensitivity to the Nagorno-Karabakh deformation microsporidia, higher in specificity and simple and convenient to operate, results are more visual and reliable, and pathogens ofall development stages exc