Optimizing method, device, equipment and medium of CHO cell culture technology

The invention discloses optimizing method, device, equipment and medium of CHO cell culture technology. The method comprises the following steps: determining a grid model of a large reactor accordingto the parameters of the space contacting with a culture solution of CHO cell in the large reactor; determining a calculation model corresponding to the grid model and the constant boundary conditionparameters of the grid model, and determining the varying boundary condition parameters of the grid model according to the technological environment of CHO cell culture; determining the Reynolds number and internal temperature in the large reactor according to the grid model, the calculation model corresponding to the grid model, and the constant boundary condition parameters and varying boundarycondition parameters of the grid model; adjusting the varying boundary condition parameters according to the relationship between the Reynolds number and a preset Reynolds number and the relationshipbetween the internal tempera