Detecting method and detecting kit for immunosuppressive agents in dried blood spots

The invention discloses a detecting method for immunosuppressive agents in dried blood spots, and further discloses a detecting kit for the immunosuppressive agents in the dried blood spots. The detecting method comprises the steps that the standard curve dried blood spots and the to-be-detected dried blood spots are mixed with an extracting solution and an internal standard solution correspondingly and then extracted, centrifuging is conducted, and supernate is taken; the supernate is blown dry and then redissolved through a redissolving solution, and detecting is conducted through a tandem mass spectrum method; according to the peak exceeding area specific value of standard samples to an internal standard object and the concentration of the standard samples, linear regression is conducted to obtain a standard curve equation of the immunosuppressive agents; the peak exceeding area specific value of to-be-detected samples to the internal standard object is substituted into the standardcurve equation, and the