Tumor vaccine based on nano-graphene oxide-Al(OH)3/antigen composite and preparation method thereof

The invention discloses a tumor vaccine based on a nano-graphene oxide-Al(OH)3/antigen composite and a preparation method thereof. The preparation method includes: 1) adding nano-graphene oxide and Al2(SO4)3 to water, stirring the mixture, regulating pH to 7-8, performing a reaction, centrifuging a product, collecting a solid and drying the solid to obtain Al(OH)3 modified nano-graphene oxide; 2)adding the Al(OH)3 modified nano-graphene oxide and antigen protein to a phosphate buffer liquid, pH being 7.4, performing incubation and centrifugation, collecting a solid, and freeze-drying the solid to obtain the tumor vaccine based on the nano-graphene oxide-Al(OH)3/antigen composite. The preparation method is simple. The tumor vaccine has high antigen protein loading capacity. On the basis ofkeeping the induction capability of the aluminum adjuvant to the humoral immune response, the tumor vaccine can further activate the cytotoxic T cell immunologic response of antigenic specificity CD8+ by means of the graphene