Liquid culture method for rapidly culturing cordyceps militaris stroma
The invention discloses a liquid culture method for rapidly culturing cordyceps militaris stroma. The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed...
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| creator | LIU SHISEN LIU TING HU JIAN JI HONG XU BING |
| description | The invention discloses a liquid culture method for rapidly culturing cordyceps militaris stroma. The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed in a culture box, and a culture vessel is placed on the magnetic stirrer; (2) culture of fruiting bodies, wherein during an after-mature period, the ambient humidity is 65% to 75% at 23-25 DEG C; during an adversity bud inducement period, the light intensity is 50-150 Lux at the temperature of 23-25DEG C, mycelia turn faint yellow after one day, and the light intensity is maintained at 100-550 Lux; during a growth period, the light intensity is maintained at 50-100 Lux at the temperature of 23-25 DEG C, the air humidity is not less than 70%, the carbon dioxide concentration is less than 0.1%, and the growth period is 2 days. According to the method, the magnetic stirrer is utilized to increase dissolved oxygen, and |
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The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed in a culture box, and a culture vessel is placed on the magnetic stirrer; (2) culture of fruiting bodies, wherein during an after-mature period, the ambient humidity is 65% to 75% at 23-25 DEG C; during an adversity bud inducement period, the light intensity is 50-150 Lux at the temperature of 23-25DEG C, mycelia turn faint yellow after one day, and the light intensity is maintained at 100-550 Lux; during a growth period, the light intensity is maintained at 50-100 Lux at the temperature of 23-25 DEG C, the air humidity is not less than 70%, the carbon dioxide concentration is less than 0.1%, and the growth period is 2 days. According to the method, the magnetic stirrer is utilized to increase dissolved oxygen, and</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED ; FISHING ; FORESTRY ; HORTICULTURE ; HUMAN NECESSITIES ; HUNTING ; TRAPPING ; WATERING</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181218&DB=EPODOC&CC=CN&NR=109006175A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181218&DB=EPODOC&CC=CN&NR=109006175A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU SHISEN</creatorcontrib><creatorcontrib>LIU TING</creatorcontrib><creatorcontrib>HU JIAN</creatorcontrib><creatorcontrib>JI HONG</creatorcontrib><creatorcontrib>XU BING</creatorcontrib><title>Liquid culture method for rapidly culturing cordyceps militaris stroma</title><description>The invention discloses a liquid culture method for rapidly culturing cordyceps militaris stroma. The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed in a culture box, and a culture vessel is placed on the magnetic stirrer; (2) culture of fruiting bodies, wherein during an after-mature period, the ambient humidity is 65% to 75% at 23-25 DEG C; during an adversity bud inducement period, the light intensity is 50-150 Lux at the temperature of 23-25DEG C, mycelia turn faint yellow after one day, and the light intensity is maintained at 100-550 Lux; during a growth period, the light intensity is maintained at 50-100 Lux at the temperature of 23-25 DEG C, the air humidity is not less than 70%, the carbon dioxide concentration is less than 0.1%, and the growth period is 2 days. According to the method, the magnetic stirrer is utilized to increase dissolved oxygen, and</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HORTICULTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>TRAPPING</subject><subject>WATERING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHDzySwszUxRSC7NKSktSlXITS3JyE9RSMsvUihKLMhMyamESmXmpSsk5xelVCanFhQr5GbmZJYkFmUWKxSXFOXnJvIwsKYl5hSn8kJpbgZFN9cQZw_d1IL8-NTigsTk1LzUknhnP0MDSwMDM0NzU0djYtQAAIrONF0</recordid><startdate>20181218</startdate><enddate>20181218</enddate><creator>LIU SHISEN</creator><creator>LIU TING</creator><creator>HU JIAN</creator><creator>JI HONG</creator><creator>XU BING</creator><scope>EVB</scope></search><sort><creationdate>20181218</creationdate><title>Liquid culture method for rapidly culturing cordyceps militaris stroma</title><author>LIU SHISEN ; LIU TING ; HU JIAN ; JI HONG ; XU BING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109006175A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HORTICULTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>TRAPPING</topic><topic>WATERING</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU SHISEN</creatorcontrib><creatorcontrib>LIU TING</creatorcontrib><creatorcontrib>HU JIAN</creatorcontrib><creatorcontrib>JI HONG</creatorcontrib><creatorcontrib>XU BING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU SHISEN</au><au>LIU TING</au><au>HU JIAN</au><au>JI HONG</au><au>XU BING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Liquid culture method for rapidly culturing cordyceps militaris stroma</title><date>2018-12-18</date><risdate>2018</risdate><abstract>The invention discloses a liquid culture method for rapidly culturing cordyceps militaris stroma. The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed in a culture box, and a culture vessel is placed on the magnetic stirrer; (2) culture of fruiting bodies, wherein during an after-mature period, the ambient humidity is 65% to 75% at 23-25 DEG C; during an adversity bud inducement period, the light intensity is 50-150 Lux at the temperature of 23-25DEG C, mycelia turn faint yellow after one day, and the light intensity is maintained at 100-550 Lux; during a growth period, the light intensity is maintained at 50-100 Lux at the temperature of 23-25 DEG C, the air humidity is not less than 70%, the carbon dioxide concentration is less than 0.1%, and the growth period is 2 days. According to the method, the magnetic stirrer is utilized to increase dissolved oxygen, and</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING TRAPPING WATERING |
| title | Liquid culture method for rapidly culturing cordyceps militaris stroma |
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