Liquid culture method for rapidly culturing cordyceps militaris stroma

The invention discloses a liquid culture method for rapidly culturing cordyceps militaris stroma. The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed...

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Bibliographische Detailangaben
Hauptverfasser: LIU SHISEN, LIU TING, HU JIAN, JI HONG, XU BING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a liquid culture method for rapidly culturing cordyceps militaris stroma. The method comprises the following steps of (1) culture of bacterium membranes, wherein cordyceps militaris strains are inoculated into a liquid culture medium for culture, a magnetic stirrer is placed in a culture box, and a culture vessel is placed on the magnetic stirrer; (2) culture of fruiting bodies, wherein during an after-mature period, the ambient humidity is 65% to 75% at 23-25 DEG C; during an adversity bud inducement period, the light intensity is 50-150 Lux at the temperature of 23-25DEG C, mycelia turn faint yellow after one day, and the light intensity is maintained at 100-550 Lux; during a growth period, the light intensity is maintained at 50-100 Lux at the temperature of 23-25 DEG C, the air humidity is not less than 70%, the carbon dioxide concentration is less than 0.1%, and the growth period is 2 days. According to the method, the magnetic stirrer is utilized to increase dissolved oxygen, and