Method for detecting free polysaccharide in Hib (Haemophilus influenzzae Type b) conjugate vaccine by sodium deoxycholate precipitation method

The invention discloses a method for detecting free polysaccharide in a Hib (Haemophilus influenzzae Type b) conjugate vaccine by a sodium deoxycholate precipitation method, and belongs to the technical field of vaccine detection. The technical scheme key points of the method are that the method comprises the following operation steps that: preprocessing a sample for test, collecting free polysaccharide solution, measuring ribose concentration, and calculating the contents of polysaccharide and the contents of the free polysaccharide in the sample for test. Firstly, saccharose is removed by anultrafiltration method, then, sodium deoxycholate is used for precipitating polysaccharide protein conjugate so as to accurately measure the contents of the polysaccharide in the Hib conjugate vaccine, and therefore, the production cost of the Hib conjugate vaccine is lowered. 本发明公开了种脱氧胆酸钠沉淀法检测Hib结合疫苗中游离多糖的方法，属于疫苗检测技术领域，其技术方案要点是，包括供试品预处理、游离多糖溶液的收集、核糖浓度的测定以及计算供试品中多糖含量以及游离多糖的含量的操作步骤，其先利用超滤法去除蔗糖，再利用脱氧胆酸钠沉淀多糖蛋白结物，从而准确的对Hib结合