Isolation plug assembly and indwelling needle without glue connection

The invention discloses an isolation plug assembly and an indwelling needle without glue connection. The indwelling needle comprises a three way needle stand, an indwelling hose arranged in the frontend of the three way needle stand, a puncture needle penetrating into the indwelling hose from the three way needle stand, the isolation plug assembly arranged in the three way needle stand, a transfusion conduit and a Y-shaped three way connecting seat, and connection between the three way needle stand and the isolation plug assembly, between the tree way needle stand and the transfusion conduitand between the transfusion conduit and the Y-shaped three way connecting seat is free of glue. A series of side effects caused by glue connection can be avoided, and due to the fact that an isolationplug has an elastic force towards the outside or from the two ends to the outside after that the puncture needle is pulled out, the isolation plug can be kept in the sealed state and resists high pressure, and blood and transf