Super-resolution imaging method, device and terminal device

The invention is applicable to the technical field of image processing, and provides a super-resolution imaging method, a device and a terminal device. The method comprises the steps of: exciting a fluorescent probe with double emission peaks on a biological sample through excitation lights, and applying an affinity reagent to the fluorescent probe after excitation; irradiating the fluorescence probe after being treated by the affinity reagent through activating lights to obtain a two-channel fluorescence intensity image; correspondingly collecting a short-wavelength signal of a short-wavelength emission peak and a long wavelength of a long-wavelength emission peak in two-channel fluorescence intensity images; selecting N-frame two-channel fluorescence intensity images, and correspondinglycalculating the fluorescence intensity ratios of the short-wavelength signal and the long-wavelength signal to obtain N proportional fluorescence images; obtaining super-resolution images through a STORM super-resolution ima