Probe combination and method suitable for physically positioning plant chromosome rDNA (ribosomal Deoxyribonucleic Acid)

The invention discloses a probe combination and method suitable for physically positioning plant chromosome rDNA (ribosomal Deoxyribonucleic Acid) and relates to the technical field of molecular cytogenetics. The probe combination comprises probes shown as SEQ ID NO. 1 to SEQ ID NO. 4 which include a 5S rDNA probe, a 5.8S rDNA probe, a 18S rDNA probe and a 25S rDNA probe respectively; the probe combination and the method are used for carrying out in-situ hybridization on plant chromosomes, can be used for capturing chromosomes with a relatively high resolution ratio and fluorescent in-situ hybridization signals, and are suitable for the in-situ hybridization of the chromosomes of various plants; the probe combination is an oligonucleotide probe and has the characteristics of low productioncost, convenience for utilization, simplicity in operation and the like. 本发明公开了种适于植物染色体rDNA物理定位的探针组合和方法；涉及分子细胞遗传学技术领域。该探针组合包括SEQ ID NO.1-4的探针，分别是5S rDNA探针、5.8S rDNA探针、18S rDNA探针和25S rDNA探针；采用该探针组合及方法进行植物染色体原位杂交，可以捕获到较高分辨率的染色