一种来自多能干细胞的神经嵴谱系周细胞及其诱导分化方法

本发明公开了一种来自多能干细胞的神经嵴谱系周细胞及其诱导分化方法，其是解离多能干细胞制备成单细胞悬液，接种在基质胶包被的孔板中，以NCN2培养基每天换液；培养一定时间后消化收集细胞；贴壁培养收集的细胞，并将培养液更换为周细胞诱导培养液，传代培养后检测周细胞的细胞表型，即得。本发明解决人脑血管周细胞来源受限及其他非限定诱导途径多能干细胞来源的周细胞异质性与混杂的缺点，获得的神经嵴谱系周细胞具有很强的维持血管稳定能力与收缩能力；标准化诱导分化程序可保证不同批次间获得的细胞群具有良好的一致性。 The invention discloses a neural crest-lineage pericyte derived from multi-potential stem cells and an induced differentiation method of the neural crest-lineage pericyte. The induced differentiationmethod comprises the following steps: dissociating the multi-potential stem cells to obtain single-cell suspension, inoculating the single-cell suspension into a matrigel-coated pore plate, and changing the suspension through an NCN2 culture medium each day; carrying out culturing for a certain period of time, carrying out digestion, and collecting cells; carrying out adherent culture on the collected cells, changing culture liquid into pericyte induced culture liquid, carrying out subculture, and detecting the cell phenotype of the pericyte. According to the induced diff