Method for culturing rhododendron fortunei polyploidy

The invention provides a method for culturing rhododendron fortunei polyploidy, and relates to the technical field of plant biology. The method comprises the following steps: sterilizing rhododendronfortunei seeds with oryzalin; transplanting the seeds to a seedling clod for culturing after the seeds are germinated, and detecting the ploidy variation of chromosome by a porosity method and a flowcytometer method; and transplanting the chromosome doubling plant determined by detection into greenhouse for culturing to obtain the polyploidy plant. The rhododendron fortunei polyploidy cultured byadopting the method has high inductivity reaching 30 percent, and high survival rate of variant plants reaching 85 percent or more; and the identifying method is simple and convenient, and can simultaneously prepare massive polyploidy materials, and the breeding efficiency is greatly improved. 本发明提供了种云锦杜鹃多倍体的培育方法，涉及植物生物技术领域，包括如下步骤：云锦杜鹃种子灭菌后，以氨磺乐灵进行处理；种子萌发后移栽至育苗块进行培养，然后通过气孔法和流式细胞仪法检测染色体倍性变化，检测确认的染色体加倍植株移栽至温室培养，即可获得多倍体植株。采用本