DNA molecule encoding chimeric Zika virus and preparation method and applications thereof

The invention belongs to the field of biological engineering, and discloses a DNA molecule encoding chimeric Zika virus and a preparation method and applications thereof. The DNA molecule contains 5'non-coding region sequences in serial connection, a coding sequence of a capsid protein C, a membrane protein precursor sequence, a coding sequence of an envelope E protein alpha missing three amino acid residues at a carboxy terminal, coding sequences of three amino acid residues at the carboxy terminal of an envelope E protein beta, a coding sequence of a non-structural protein NS and a 3' non-coding region sequence; the membrane protein precursor sequence and the coding sequence of the envelope E protein alpha root in Zika viruses; and the rest sequences root in Japanese encephalitis virusattenuated strains. A cDNA sequence corresponding to a genomic RNA of chimeric virus is the same with the sequence of the DNA molecule. The DNA molecule and/or chimeric viruses has good application prospects on preventing Zika