Method for rapid detection of aflatoxins in medicinal material and test paper card used in method

The invention relates to a method for rapid detection of aflatoxins in a medicinal material and a test paper card used in the method. The method includes the following steps: providing a colloidal gold test paper card for aflatoxin detection; extracting a sample with an acetonitrile solution, applyi...

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Hauptverfasser: FU XINTONG, CHEN YOUGEN, YANG WENLIANG, FAN MIAOXUAN, GUO HONGZHU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for rapid detection of aflatoxins in a medicinal material and a test paper card used in the method. The method includes the following steps: providing a colloidal gold test paper card for aflatoxin detection; extracting a sample with an acetonitrile solution, applying the sample to a NH2/C18 column for purifying, applying the sample to a C18 column for secondary,transferring the sample extract, applying the sample extract to the colloidal gold test paper card, performing incubation at constant temperature of 45DEG C, measuring test strip reading by a reader,and calculating the content of the aflatoxins in the medicinal material according to sample loading quantity and sample diluting time. The method can detect results in 5 minutes, and the method can accurately and quantitatively detect various root and rhizome traditional Chinese medicines. The test paper card has the characteristics of simple operation, high sensitivity, fast detection speed, accurate detection result and