Lead ion fluorescence detection method and nanoparticle fluorescence sensor for lead ion detection
The invention discloses a lead ion fluorescence detection method and a nanoparticle fluorescence sensor for lead ion detection. The preparation steps comprise: (1) separately synthesizing nanoparticles and gold nanoparticles; (2) respectively modifying the nanoparticles and the gold nanoparticles wi...
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| creator | FAN JIANWEI RAN XIANQIANG SUN YU LIU NINGTAO DENG WEIJIAN ZHANG HUA XU HUAWEI LIAO ZHENLIANG LYU MENGHUA |
| description | The invention discloses a lead ion fluorescence detection method and a nanoparticle fluorescence sensor for lead ion detection. The preparation steps comprise: (1) separately synthesizing nanoparticles and gold nanoparticles; (2) respectively modifying the nanoparticles and the gold nanoparticles with two different DNA single strands, wherein the two DNA single strands are partially complementaryand matched to each other; (3) incubating the DNA1-modified nanoparticles and the DNA2-modified gold nanoparticles in a buffer solution for 90 min to obtain a hybridization solution; (4) adding different concentrations of lead ions to the hybridization solution to unwind the DNA double-strand the nanoparticles and the gold nanoparticles; (5) measuring the fluorescence of the system at a room temperature by using a fluorescence spectrometer; and (6) according to the fluorescence intensity signal, recording the test results. According to the present invention, the high sensitivity of fluorescence and the specificity of |
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The preparation steps comprise: (1) separately synthesizing nanoparticles and gold nanoparticles; (2) respectively modifying the nanoparticles and the gold nanoparticles with two different DNA single strands, wherein the two DNA single strands are partially complementaryand matched to each other; (3) incubating the DNA1-modified nanoparticles and the DNA2-modified gold nanoparticles in a buffer solution for 90 min to obtain a hybridization solution; (4) adding different concentrations of lead ions to the hybridization solution to unwind the DNA double-strand the nanoparticles and the gold nanoparticles; (5) measuring the fluorescence of the system at a room temperature by using a fluorescence spectrometer; and (6) according to the fluorescence intensity signal, recording the test results. 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| title | Lead ion fluorescence detection method and nanoparticle fluorescence sensor for lead ion detection |
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