Preparation method of poultry vaccine dilution liquid

The invention provides a preparation method of a poultry vaccine dilution liquid, and belongs to the technical field of biomedicine. The preparation method comprises: mixing chicken bursa, chicken intestine and water, homogenizing, carrying out repeated freezing-thawing on the obtained homogenized liquid, mixing the obtained freezing-thawing liquid and acetic acid, sequentially carrying out stirring and centrifugation, mixing the obtained centrifugation supernatant and beta-propiolactone, inactivating, hydrolyzing the obtained inactivated liquid for 1-3 h at a temperature of 35-42 DEG C, carrying out micro-filtration on the obtained hydrolyzate, adjusting the pH value of the obtained micro-filtrate, carrying out ultra-filtration on the obtained adjusted liquid, and sterilizing the obtainedultra-filtrate to obtain the poultry vaccine dilution liquid. According to the present invention, the poultry vaccine dilution liquid prepared through the preparation method can completely kill the non-specific pathogenic ba