Simple and fast preparation and operation technology for virus-free sweet potato seedlings

The invention discloses a simple and fast preparation and operation technology of virus-free sweet potato seedlings, the simple and fast preparation and operation technology has a low threshold and does not need an anatomical lens and a professional technicians to peel off stem tips, and can be oper...

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Bibliographische Detailangaben
Hauptverfasser: HUANG YANXIA, GUO QIMAO, LIN JINHU, LIN ZILONG, LIAO FUQIN, CHEN GENHUI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a simple and fast preparation and operation technology of virus-free sweet potato seedlings, the simple and fast preparation and operation technology has a low threshold and does not need an anatomical lens and a professional technicians to peel off stem tips, and can be operated by general technicians and the most basic tissue culture room conditions; the stem tips of aseptic seedlings are used, no disinfection step is needed, and so long as properly operated, the stem tips may not be polluted basically; steps are simplified, and after the stem tips are inoculated to an inductive differentiation medium, callus formation is performed first, rooting is then performed, and the seedlings are formed without transferring of the medium for many times. Variant materials are easy to produce by formation of the seedlings by callus induction, and new breeding materials are provided for breeders for variety breeding. 本发明公开了种简易快捷甘薯脱毒苗的制备操作技术,该技术的门槛低,不需要解剖镜和专业技术人员来剥离茎尖,般的技术工人和最基本的组培室条件即可操作;使用的是无菌苗的