Efficient liquid- phase detection method for L-tyrosine

The invention relates to an efficient liquid-phase detection method for L-tyrosine. A chromatographic condition is as follows: an octadecyl bonded silica gel chromatographic column is adopted, a mixedsolution of phosphate buffer and methanol is taken as a flowing phase, the volume ratio of the phosphate buffer to methanol is 6: (3.5-4.5), the detection wavelength is 220-230 nm, the column temperature is 25-35 DEG C, and the flow rate of the flowing phase is 0.7-1.0 mL/min. The method provided by the invention can be used for measuring the content of L-tyrosine quickly and accurately, can reduce errors, ensure that the quality is controllable, and is simple to operate, low in detection cost, short in detection time and high in detection efficiency. 本发明涉及种L-酪氨酸的高效液相检测方法，色谱条件为：采用十八烷基硅烷键合硅胶色谱柱；以磷酸盐缓冲液与甲醇的混合溶液作为流动相，且磷酸盐缓冲液与甲醇的体积比为6∶3.5～4.5；检测波长为220～230nm；柱温为25～35℃；流动相流速：0.7～1.0mL/min。本发明方法可快速、准确地测定出L-酪氨酸的含量，能够减小误差，确保其质量可控，并且操作简单、检测成本低、检测时间短、检测效率高。