BSA-based super-resolution imaging probe as well as preparation method and application thereof

The invention discloses a BSA-based super-resolution imaging probe as well as a preparation method and application thereof. The super-resolution imaging probe is BSA nanometer balls coated with AlexaFluor 594 dye; in addition, HER2 antibodies are coupled on the surface. The probe has the fluorescence flickering features and can be used for super-resolution unimolecule imaging. In addition, the probe can be used for targeting high-EHR2-expression tumor cells and can enter the cells through receptor-mediated endocytosis; through an SMLM imaging technology, the tumor cells after the probe intakecan be subjected to super resolution optical imaging. The application of the super-resolution imaging probe in the cells to super-resolution imaging can be realized; an SMLM microscope is used; the distribution of the probe in the cells can be precisely positioned. 本发明公开了种基于BSA的超分辨成像探针及其制备方法和应用，该超分辨成像探针为包裹了Alexa Fluor 594染料的BSA纳米球，并且其表面偶联了HER2抗体；该探针具有荧光闪烁特性，可用于超分辨单分子定位成像；另外，该探针能靶向高表达HER2的肿瘤细胞，并能通过受体介导的内吞作用进入细胞；通过SMLM成像技术即