High-throughput rapid antimicrobial susceptibility test kit for veterinary clinical application

The invention discloses a high-throughput rapid antimicrobial susceptibility test kit for veterinary clinical application and a method. The kit comprises an independently-packaged enrichment medium, an antimicrobial susceptibility test plate and reaction liquid; the enrichment medium is selected fro...

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Hauptverfasser: HE CHENGHUA, FAN YANHONG, HU RENLI, ZHANG XUEMING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a high-throughput rapid antimicrobial susceptibility test kit for veterinary clinical application and a method. The kit comprises an independently-packaged enrichment medium, an antimicrobial susceptibility test plate and reaction liquid; the enrichment medium is selected from a sterile 1640 medium or an MH medium with 1% sterile neonatal bovine serum and 0.1% sterile NAD added; the antimicrobial susceptibility test plate is a reaction plate coated with a plurality of antibiotics, each hole of the reaction plate is coated with an antibiotic, and the concentration of theantibiotics is 1-2 times of the maximum plasma concentration after the clinical administration of the antibiotics; and the reaction liquid is a 0.2% resazurin sterile solution. According to the kit and the method, an antimicrobial susceptibility test is carried out, the time for the result is short, more drugs are tested at a time, the kit and the method are easy to operate, and special instruments and equipment are not