Amino lyase mutant protein and coding gene and application thereof

The invention discloses amino lyase mutant protein and a coding gene and application thereof. The protein is obtained by conducting mutation on at least one of 187-326 site amino acid residue shown inthe sequence 2 of the sequence table. The invention further discloses a method for utilizing the protein to prepare (R) 1-propyl(2-amino)formic acid. The protein provided by the invention has the relatively high amino lyase activity, and meanwhile can catalyze an ammonification reaction which taking trans-2-butenoic acid as a substrate to produce (R) 1-prypyl(2-amino)formic acid, the yield is high, the requirements of 100% stereoscopic selectivity is satisfied, and the protein has the very wide application prospects. 本发明公开了种氨基裂解酶突变体蛋白及其编码基因与应用。本发明保护的蛋白质，是将序列表中序列2所示的蛋白质第187-326位氨基酸残基中的至少个进行突变得到的。本发明还保护利用所述蛋白质制备(R)1-丙基(2-氨基)甲酸的方法。本发明所提供的蛋白质具有较高的氨基裂解酶活性，同时可以催化以反式2-丁烯酸为底物的加氨反应，生产(R)1-丙基(2-氨基)甲酸，产率高且满足100％立体选择性的要求，具有非常广阔的应用前景。