Culture medium of umbilical cord mesenchymal stem cells

The invention provides a culture medium of umbilical cord mesenchymal stem cells. The culture medium comprises a DMEM (Dulbecco's Modified Eagle Medium)/F12 culture medium, an adding component added into the DMEM/F12 culture medium and ivy extract, wherein the adding component is prepared from...

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Hauptverfasser: ZHANG HONG, DONG ZHENLEI, ZHANG BINGQIANG, QI FENGCHUN, YU MIAO, GONG LI, JIN DUO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a culture medium of umbilical cord mesenchymal stem cells. The culture medium comprises a DMEM (Dulbecco's Modified Eagle Medium)/F12 culture medium, an adding component added into the DMEM/F12 culture medium and ivy extract, wherein the adding component is prepared from the following components: human serum albumin, transferrin, a vascular endothelial growth factor, a basicfibroblast growth factor, arginine, a leukemia inhibitory factor, a stem cell factor, beta cytokine, parathyroid hormone, a platelet-derived growth factor, a tumor necrosis factor alpha, interleukin,erythropoietin, thrombopoietin, alpha-D-glucose, vitamin C, resveratrol, NaHCO3 and ganglioside; the ivy extract is liquid and is extracted from leaves of ivy through a conventional method; the finalconcentration is 20 to 50mg/L after the ivy extract is added. By adopting the culture medium, the problems in the prior art are solved, and the cells can keep good state and proliferation speed. 种脐带间充质干细胞的培养基,包括DMEM/F12培养基、添加在