Method of simultaneously extracting ursolic acid and oleanolic acid in herba hedyotidis diffusae by sub-critical water

The invention discloses a method of simultaneously extracting ursolic acid and oleanolic acid in herba hedyotidis diffusae by sub-critical water and belongs to the technical field of extraction and separation of natural active ingredients, by taking herba hedyotidis diffusae as raw material, firstly, the impact of grain size, extraction temperature and time, liquid-solid ratio and extraction pressure on extraction amount of ursolic acid (UA) and oleanolic acid (OA) is tested by a single factor testing method and optimal conditions are determined in a surface response method; extracting solution is collected, a crude product is produced through rotary evaporation and a petroleum ether degreasing process; ultimately, ursolic acid and oleanolic acid are produced through the techniques of acidand alkali treatment, macroporous resin absorption and rotary evaporation and concentration and the like. The method of simultaneously extracting ursolic acid and oleanolic acid in herba hedyotidis diffusae by sub-critical w