Method for preparing quercetin-3-O-glucose rhamnoside glucoside from tea leaves

The invention discloses a method for preparing quercetin-3-O-glucose rhamnoside glucoside from tea leaves, comprising the following steps: (1), extracting tea leaves with water and filtering to obtaina tea extract; (2), performing polyamide column chromatography on the tea extract to obtain a crude product solution, wherein the gradient elution conditions are: water, 15 percent of aqueous methanol solution, crude solvent extraction solvent, and pure methanol, an eluant collected by elution of a crude extract solvent is concentrated to obtain a crude product solution; (3), performing preparative chromatography on the crude product solution, and collecting the components to obtain a quercetin-3-O-glucose rhamnoside glucoside solution; (4) concentrating the solution obtained in step (3) under reduced pressure, and freeze drying to obtain the quercetin-3-O-glucose rhamnoside glucoside. The compound can be separated and purified from a large amount of tea leaves by the method of the invention. 本发明公开了种从茶叶中制备槲皮素-3-O