Arabidopsis gene mutant identifying method

The invention discloses an Arabidopsis gene mutant identifying method which includes the steps: designing a primer containing a restriction endonuclease digestion site by a dCAPs Finder 2.0 website; performing primer synthesis and PCR (polymerase chain reaction) amplification to amplify a nucleic acid fragment containing a restriction endonuclease digestion site; cutting out fragments with different sizes of a wild type and a mutant through restriction endonuclease digestion; distinguishing sizes of stripes in agarose gel by gel electrophoresis; analyzing that a sample gene is normal and mutant or heterozygous by a nucleic acid electrophoresis imaging system. The Arabidopsis gene mutant identifying method is high in repeatability, the work time of the method is only 30% or less that of a traditional identifying method, and the identifying cost of each sample is reduced by 80% or more. 本发明公开了种拟南芥基因突变体的鉴定方法，包括以下步骤：通过dCAPs Finder 2.0网站，设计含有限制性核酸内切酶酶切位点的引物；然后进行引物合成和PCR扩增，扩增出含有限制性核酸内切酶酶切位点的核酸片段；通过限制性核酸内切酶酶切，切出野生型、