Method for constructing white-eye strain of bactrocera dorsalis

The invention discloses a method for constructing a white-eye strain of bactrocera dorsalis. Cas9 mRNA is mixed with a target gene gRNA, the mixture is injected into an embryo by microinjection, and then the white-eye strain of bactrocera dorsalis is obtained by genetic hybridization. According to the method, the best research steps and technical parameters are considered, a gene editing method for the white-eye strain of bactrocera dorsalis is constructed, the stably genetically pure white-eye strain of bactrocera dorsalis is acquired quickly and accurately, the technical blank is filled, ideal experimental materials and research ideas are provided for biological research of bactrocera dorsalis, and the method has good application prospects. 本发明公开了种构建桔小实蝇白眼品系的方法，将Cas9 mRNA与靶基因gRNA混合后，利用显微注射的方式，注射到胚胎中，再经过遗传杂交获得桔小实蝇白眼品系。本发明考虑了最佳的研究步骤和技术参数，构建了桔小实蝇白眼品系的基因编辑方法，快速、准确地获得稳定遗传的纯合桔小实蝇白眼品系，填补了技术空白，为桔小实蝇的生物学研究提供了理想的实验材料及研究思路，具有良好的应用前景。