Bone accellular stent-based human multiple myeloma micro-environment model building method

The invention discloses a bone accellular stent-based human multiple myeloma micro-environment model building method, which comprises steps of preparation of a bone accellular stent, evaluation of anaccellular effect of the bone accellular stent, cobalt-60 radiation for sterilization, inoculation of...

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Hauptverfasser: GUO YIBING, SHEN XIANJUAN, YAO JUNZHONG, GU ZHIFENG, LI XIAOHONG, JU SHAOQING, LU JINGJING, ZHU HUI, QI JING, XU LIANCHENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a bone accellular stent-based human multiple myeloma micro-environment model building method, which comprises steps of preparation of a bone accellular stent, evaluation of anaccellular effect of the bone accellular stent, cobalt-60 radiation for sterilization, inoculation of human multiple myeloma cells and the like. The invention provides the human multiple myeloma micro-environment model building method to solve the problem that a current multiple myeloma model can hardly simulate a real environment in the body, so that a cell model is provided for further researchon multiple myeloma diseases. 本发明公开了种基于骨脱细胞支架的人多发性骨髓瘤微环境模型的构建方法,包括骨脱细胞支架制备、骨脱细胞支架脱细胞效果评价、钴辐照灭菌、人多发性骨髓瘤细胞接种等步骤。本发明提供了种人多发性骨髓瘤微环境模型的建立方法,解决目前多发性骨髓瘤模型难以模拟体内真实环境的问题,为多发性骨髓瘤疾病的进步研究提供了种细胞模型。