Preparation method of culture medium solution containing cholesterol micelle

Preparation method of culture medium solution containing cholesterol micelle

The invention discloses a preparation method of a culture medium solution containing cholesterol micelle. The preparation method comprises the following steps: (1) weighing cholesterol and putting thecholesterol into a test tube; adding cow bile salt, sucrose fatty acid ester and Tween-80; transferr...

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Hauptverfasser: XIA FENGGENG, LIN WEIFENG, BAO ZHINING, HE SHANSHAN
Format: Patent
Sprache:chi ; eng
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator XIA FENGGENG
LIN WEIFENG
BAO ZHINING
HE SHANSHAN
description The invention discloses a preparation method of a culture medium solution containing cholesterol micelle. The preparation method comprises the following steps: (1) weighing cholesterol and putting thecholesterol into a test tube; adding cow bile salt, sucrose fatty acid ester and Tween-80; transferring absolute ethyl alcohol into the test tube; carrying out water-bath heating; then carrying out vortex treatment by adopting a vortex mixer, so as to obtain a crude mixed solution; (2) treating the crude mixed solution by utilizing ultrasonic waves and carrying out vortex blending every other 4 to 8 minutes; filtering and removing bacteria by utilizing a micro-pore filtration membrane, so as to obtain a cholesterol micelle solution; (3) adding the cholesterol micelle solution into a fermentation culture medium while the solution is hot; uniformly stirring by utilizing a magnetic stirrer; placing for 72 hours or more until no cholesterol precipitation phenomenon exists. The preparation method disclosed by the inve
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The preparation method comprises the following steps: (1) weighing cholesterol and putting thecholesterol into a test tube; adding cow bile salt, sucrose fatty acid ester and Tween-80; transferring absolute ethyl alcohol into the test tube; carrying out water-bath heating; then carrying out vortex treatment by adopting a vortex mixer, so as to obtain a crude mixed solution; (2) treating the crude mixed solution by utilizing ultrasonic waves and carrying out vortex blending every other 4 to 8 minutes; filtering and removing bacteria by utilizing a micro-pore filtration membrane, so as to obtain a cholesterol micelle solution; (3) adding the cholesterol micelle solution into a fermentation culture medium while the solution is hot; uniformly stirring by utilizing a magnetic stirrer; placing for 72 hours or more until no cholesterol precipitation phenomenon exists. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Preparation method of culture medium solution containing cholesterol micelle
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