Preparation method of culture medium solution containing cholesterol micelle

The invention discloses a preparation method of a culture medium solution containing cholesterol micelle. The preparation method comprises the following steps: (1) weighing cholesterol and putting thecholesterol into a test tube; adding cow bile salt, sucrose fatty acid ester and Tween-80; transferring absolute ethyl alcohol into the test tube; carrying out water-bath heating; then carrying out vortex treatment by adopting a vortex mixer, so as to obtain a crude mixed solution; (2) treating the crude mixed solution by utilizing ultrasonic waves and carrying out vortex blending every other 4 to 8 minutes; filtering and removing bacteria by utilizing a micro-pore filtration membrane, so as to obtain a cholesterol micelle solution; (3) adding the cholesterol micelle solution into a fermentation culture medium while the solution is hot; uniformly stirring by utilizing a magnetic stirrer; placing for 72 hours or more until no cholesterol precipitation phenomenon exists. The preparation method disclosed by the inve