DC targeted nano vaccine applied to immunotherapy of liver cancer and preparation method thereof

The invention relates to a DC targeted nano vaccine applied to immunotherapy of liver cancer and a preparation method thereof, which are characterized in that mannose modified ethylene glycol chitosanis used as a carrier material, a liver cancer specific polypeptide antigen phosphatidylinositol proteoglycan-3144-152 peptide (GPC-3144-152, FVGEFFTDV) is an immunizing antigen, and the nano vaccineis prepared by electrostatic interaction. The nano vaccine can increase the antigen endocytosis quantity of dendritic cell (DC), protect antigen from rapid degradation and clearance, prolong the action time of antigen, and stimulate the body to produce an effective immune response. The raw materials of the nano vaccine are cheap and easily available, the preparation method is simple and easy to repeat, the large-scale processing and production are simple, the immunotherapy effect of liver cancer can be enhanced, and therefore, the DC targeted nano vaccine has good application prospects. 本发明涉及种应用于肝癌免疫治疗的DC靶向性纳米疫苗及其制备方法，