Determination method for activity of millet enzyme
The invention discloses a determination method for the activity of millet enzyme, and relates to the technical field of agricultural biology. The method comprises the following steps: setting the 12thday of the millet flowering stage as the first day, and sequentially and respectively collecting you...
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| creator | BAI YUTING LI XINGCONG WANG CANGUAN GUO SHIHUA LI QIANG REN QINYONG LI TAO GAO YUAN |
| description | The invention discloses a determination method for the activity of millet enzyme, and relates to the technical field of agricultural biology. The method comprises the following steps: setting the 12thday of the millet flowering stage as the first day, and sequentially and respectively collecting young ears as to-be-detected samples on the 3rd day, the 5th day, the 10th day, the 15th day, the 20thday, the 25th day and the 30th day; grinding the to-be-detected samples by utilizing liquid nitrogen, weighing the to-be-detected samples with preset weight and purifying to-be-tested enzyme by a kit; determining the activity of the to-be-tested enzyme by an ultraviolet spectrophotometer to obtain enzyme activity data of millet. The method for determining the activity of the enzyme, disclosed bythe invention, has the advantages of accurate result and simple operation, and can provide a material basis for transcriptome sequence analysis of round-grain glutinous millet endosperm formation phase and screening differentia |
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The method comprises the following steps: setting the 12thday of the millet flowering stage as the first day, and sequentially and respectively collecting young ears as to-be-detected samples on the 3rd day, the 5th day, the 10th day, the 15th day, the 20thday, the 25th day and the 30th day; grinding the to-be-detected samples by utilizing liquid nitrogen, weighing the to-be-detected samples with preset weight and purifying to-be-tested enzyme by a kit; determining the activity of the to-be-tested enzyme by an ultraviolet spectrophotometer to obtain enzyme activity data of millet. 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The method comprises the following steps: setting the 12thday of the millet flowering stage as the first day, and sequentially and respectively collecting young ears as to-be-detected samples on the 3rd day, the 5th day, the 10th day, the 15th day, the 20thday, the 25th day and the 30th day; grinding the to-be-detected samples by utilizing liquid nitrogen, weighing the to-be-detected samples with preset weight and purifying to-be-tested enzyme by a kit; determining the activity of the to-be-tested enzyme by an ultraviolet spectrophotometer to obtain enzyme activity data of millet. The method for determining the activity of the enzyme, disclosed bythe invention, has the advantages of accurate result and simple operation, and can provide a material basis for transcriptome sequence analysis of round-grain glutinous millet endosperm formation phase and screening differentia</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Determination method for activity of millet enzyme |
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