Determination method for activity of millet enzyme

The invention discloses a determination method for the activity of millet enzyme, and relates to the technical field of agricultural biology. The method comprises the following steps: setting the 12thday of the millet flowering stage as the first day, and sequentially and respectively collecting young ears as to-be-detected samples on the 3rd day, the 5th day, the 10th day, the 15th day, the 20thday, the 25th day and the 30th day; grinding the to-be-detected samples by utilizing liquid nitrogen, weighing the to-be-detected samples with preset weight and purifying to-be-tested enzyme by a kit; determining the activity of the to-be-tested enzyme by an ultraviolet spectrophotometer to obtain enzyme activity data of millet. The method for determining the activity of the enzyme, disclosed bythe invention, has the advantages of accurate result and simple operation, and can provide a material basis for transcriptome sequence analysis of round-grain glutinous millet endosperm formation phase and screening differentia