G72 protein and three mutant proteins thereof and preparation and purification methods and application thereof

The invention provides a G72 protein and three mutant proteins thereof, namely G72-6H, G72R30A-6H, G72R30D-6H and G72R30K-6H, wherein the genes of the three mutant proteins G72R30A-6H, G72R30D-6H andG72R30K-6H are Arg30 of the G72 gene mutated into Ala, Asp and Lys respectively. The preparation and purification methods comprise the following steps: step 1, preparation of plasmids; step 2, expression of proteins; and step 3, purification of the proteins. A G72 protein with higher purity and three mutant proteins thereof are obtained by utilizing the molecular biological technique, can be applied to a series of studies on the pathology of schizophrenia, are favorable for being developed into reagents for treating mental diseases and diagnosing and detecting mental diseases, and have wide application prospects. 本发明提出了种G72蛋白及其三种突变体蛋白，分别为G72-6H、G72-6H、G72-6H、G72-6H，其特征在于，三种突变体蛋白G72-6H、G72-6H、G72-6H的基因为G72基因的Arg30分别突变成Ala、Asp及Lys。其制备和纯化方法包括以下步骤：步骤、质粒制备；步骤二、蛋白质的表达；步骤三、蛋白的纯化。本发明利用分子生物学技术得到纯度较高的G72蛋白以及其三种突变体蛋白，可将其应用于精