In-vitro culture and aseptic grafting method of excellent tomatoes

The invention belongs to the field of agricultural production and particularly relates to an in-vitro culture and aseptic grafting method of excellent tomatoes. The method includes the steps of that 1), effective rootless seedlings in bottles, of tomato varieties with excellent quality are obtained through induction and proliferation culture of stem segments; 2), rootstock in the bottles are obtained by induction culture and seedling strengthening of seeds of the excellent tomato rootstock varieties; 3), the effective rootless seedlings are grafted onto the rootstock in the bottles, and grafted seedlings with stable growth are obtained via culture and grafted for field planting. The culture method is low in cost and high in total yield of the tomatoes. 本发明属于农业生产领域，具体涉及种优良番茄离体培养及无菌嫁接方法。包括以下步骤：（1）具有优良品质的番茄品种通过茎段诱导增殖获得瓶内有效无根苗；（2）将优良番茄砧木品种种子经诱导培养、壮苗得到瓶内砧木；（3）将有效无根苗嫁接在瓶内砧木上，经过培养后，得到生长稳定的嫁接苗，移栽到田间定植。该培养方法成本低，番茄总产量高。