NIPT (Non-Invasive Prenatal Testing) GC content calibration method and device and computer readable storage medium

The invention relates to a NIPT (Non-Invasive Prenatal Testing) GC content calibration method and device and a computer readable storage medium. The method comprises the steps of: calculating GC content of a to-be-tested sample; according to the GC content of the to-be-tested sample, selecting a sample of which a difference from the to-be-tested sample in GC content is within a preset range from apreset reference library, and constructing a new reference library; and according to the new reference library, carrying out intra-sample GC content calibration and Z value calculation on the to-be-tested sample. According to the scheme, a quality control unqualified ratio can be effectively reduced. 种NIPT的GC含量校准方法、装置及计算机可读存储介质，所述方法包括：计算待测试样本的GC含量；根据所述待测试样本的GC含量，从预设的参考库中选取与所述待测试样本的GC含量的差值处于预设范围内的样本，构建新参考库；根据所述新参考库，对所述待测试样本进行样本内GC含量校准及Z值计算。上述方案能够有效降低质控不合格比例。