SKA3 gene interference lentivirus carrier and preparation method thereof

The invention relates to the technical field of genetic engineering, in particular to an SKA3 gene interference lentivirus carrier and a preparation method thereof. According to the SKA3 gene interference lentivirus carrier, the carrier is a shRNA template sequence of a humanized U6 promoter transcription targeting human SKA3 gene. The preparation method comprises the following steps that A1, AgeI and EcoR I are used for conducing digestion on PLKO. 1puro skeleton carrier vectors sequentially, gel electrophoresis recovery is conducted, and 3 different shRNA template DNA sequences of the targeting human SKA3 gene and the PLKO. 1puro skeleton carrier vectors recovered through gel electrophoresis are connected to obtain 3 connection products; A2, 3 kinds of bacterial solutions are taken andused for preparing three kinds of transformed bacterial solutions; A3, three LB agar plates are taken, and one transformed bacterial solution is added onto each LB agar plate for overnight culture; 4,monoclonal bacteria are pi