Method for preparing duck tembusu virus inactivated vaccine and duck tembusu virus inactivated vaccine

The invention discloses a method for preparing a duck tembusu virus inactivated vaccine and the duck tembusu virus inactivated vaccine. A cell line used for virus inoculation is EB66 cell line (a duckembryonic stem cell-derived cell strain), and a bioreactor is adopted for virus culture in a serum-f...

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Hauptverfasser: WANG XIAOLEI, YANG YUN, LUO SHUN, ZHANG YEXIN, GENG FENGTING, CHEN PEILAN, QIAN JIANNING, YANG GUIJUN, WANG MENG, LIN JIAN, LIU LIXIN, LIU YUEHUAN, PAN JIE, DUAN HUIJUAN, WANG JIAQI, CHENG HUIMIN, YANG ZHIYUAN, ZHAO ZHIXIN, ZHAO JICHENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for preparing a duck tembusu virus inactivated vaccine and the duck tembusu virus inactivated vaccine. A cell line used for virus inoculation is EB66 cell line (a duckembryonic stem cell-derived cell strain), and a bioreactor is adopted for virus culture in a serum-free full suspension manner; and the method comprises the following steps: 1) breeding of virus species; 2) establishment of virus seed batches; 3) preparation of cell venom; 4) inactivation of viruses; and 5) emulsification and other steps to complete the preparation of the vaccine. The method provided by the invention has the characteristics that the prepared cell venom is high in virus content, the production process is stable, intelligent control is achieved, large-scale serum-free suspension culture is realized, the operation is easy, and the cost is low; and the prepared duck tembusu virus inactivated vaccine has the advantages of safety, low side reactions, high immune efficacy, smallbatch-to-batch difference