Tissue culture rapid breeding technology for notopterygium incisum seeds
The invention discloses a tissue culture rapid breeding technology for notopterygium incisum seeds. The tissue culture rapid breeding technology comprises the following steps: inoculating the notopterygium incisum seeds subjected to stratifcation treatment to a basal culture medium to obtain 50 perc...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a tissue culture rapid breeding technology for notopterygium incisum seeds. The tissue culture rapid breeding technology comprises the following steps: inoculating the notopterygium incisum seeds subjected to stratifcation treatment to a basal culture medium to obtain 50 percent or above of induced germination rate; subsequently, transferring buds to a proliferation culturemedium and enabling a proliferation system to be improved to about 6, wherein plants have no malformation or browning; finally, segmenting a proliferation body into a single plant for rooting culture. According to the tissue culture rapid breeding technology disclosed by the invention, the notopterygium incisum seeds are adopted to replace radical buds or young leaves of notopterygium incisum toserve as explants for tissue culture, and the influence of ambient temperature on explant culture is eliminated; in addition, the proliferation efficiency of the explants is remarkably improved, and thereby rapid breeding of n |
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