Specific primer, method and kit for detecting and identifying Wolbachia infection in insect body

The invention discloses a specific primer, a method and a kit for detecting and identifying Wolbachia infection in an insect body. The specific amplification primer Tw16s1 comprises a forward primer Fand a reverse primer R, wherein sequences thereof are as shown in SEQ ID NO: 1-2. The detection method comprises the following steps: S1, extracting total DNA of an insect to be detected; S2, using the DNA in the step S1 as a template, performing a PCR amplified reaction by using the primer; S3, performing electrophoretic determination on a PCR amplified reaction product in the step S2, and judging. The primer is strong in specificity, the amplification product is a single target fragment, and the sensitivity is high, false-negative and non-specific samples can be screened, so a detection rate is improved. A process from the DNA extraction to the result acquisition only takes about 4 hours. The sample can be accurately and rapidly detected whether to be infected by Wolbachia, and a foundation is provided for the